Biobase 4 Channel 510~730nm Real-Time Fluorescent Quantitative PCR Dtection System

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Product Description

Overview & Basic Info

Model NO.
LEIA-X4
Certification
CE
Reaction System
4
Detector
MPPC
Detection Wavelength
510~730nm
Scanning Mode
Full Plate Scanning
Sample Capacity
96X0.1ml PCR Plate
Excitation Wavelength
455~680nm
Channel
4
Warranty
12 Months
HS Code
900120000

Product Description

Real-Time Fluorescent Quantitative PCR Detection System

Real-Time PCR System

Introduction:

Real-time PCR is used for sensitive, specific detection and quantification of nucleic acid targets. This system utilizes powerful assay design algorithms, optimized qPCR reagents, and intuitive data analysis software to harness the power of qPCR across diverse applications.

Working Principle:

Temperature step changes are controlled by semiconductor Peltier elements to achieve PCR amplification. High-sensitivity MPPC units detect fluorescence via top-scanning non-contact excitation. Motor-controlled X and Y axis movement enables 96-well scanning followed by accurate software analysis.

Key Features:

  • Excellent temperature control with max ramp rate ≥5.0ºC/s.
  • No edge effect and no optical path correction required due to top excitation/detection.
  • MPPC sensor for high-sensitivity fluorescence detection.
  • Long-life maintenance-free LED light source with stable emission.
  • 4-channel detection with zero cross-interference.
  • User-friendly software with comprehensive reporting and parameter storage.

Product Parameters

Product NameFluorescence Quantitative PCR Detection System
ModelLEIA-X4
Sample Capacity96x0.1ml PCR plate, 12x8-strip tubes, 96*0.1ml single tube
Reaction System10~50μl
Dynamics Range1-10^10 copies
DetectorMPPC
Detection PathF1: FAM/SYBRF2: VIC/JOE/HEXF3: ROX/TEXAS-REDF4: Cy5
Excitation Range455~680nm
Detection Range510~730nm
Ramp Rate5.0ºC/s (max)
Temp. Accuracy±0.3ºC
Scanning Time8.5s
Operation SystemWindows 10
Dimensions (mm)375*490*365

Packaging & Logistics

Packaging

Our Advantages

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Frequently Asked Questions

Q1: What is the maximum heating and cooling rate of the LEIA-X4?
The system features excellent temperature control with a maximum ramp rate of ≥5.0ºC/s.
Q2: How many channels are available for fluorescence detection?
It provides 4-channel fluorescence detection (F1-F4) covering dyes like FAM, SYBR Green, VIC, JOE, ROX, and Cy5.
Q3: What type of detector does this PCR system use?
The system utilizes a high-sensitivity MPPC (Multi-Pixel Photon Counter) unit for precise fluorescence detection.
Q4: Does the system require optical path correction?
No, the top excitation and detection structure ensures non-contact measurement with no edge effects, eliminating the need for optical path correction.
Q5: What is the sample capacity for a single run?
The system supports a 96x0.1ml PCR plate, 12x8-strip tubes, or 96 individual 0.1ml single tubes.
Q6: What software capabilities are included?
The software supports absolute quantification, relative quantification, SNP analysis, and melting curve analysis on Windows 10.

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