Introduction Real-time PCR is used for sensitive, specific detection and quantification of nucleic acid targets. Powerful assay design algorithms, optimized qPCR reagents, and flexible instrumentation help harness the power of qPCR across a rich and diverse set of applications. Application It can be widely used for Infectious disease research, Food pathogen detection, Waterborne pathogen detection, Pharmaceutical analytics, Stem cell research, Pharmacogenomics research, Oncology and genetic disease research, Plant sciences and agricultural biotechnology. Working Principle The temperature step change is controlled by the semiconductor peltier to realize PCR amplification. Use high-sensitivity MPPC unit to detect fluorescence; Program control channel switching, non-contact excitation/detection on the top structure, coordinated with motor control X and Y axis movement to achieve 96-hole scanning.